Congratulations to Dr. Anastasiia Misiura for successfully defending her Ph.D. thesis entitled, “2D and 3D Single-Molecule Microscopy to Enhance Protein Chromatography” in Summer 2022!
To advance our understanding of underlying phenomena in a chromatographic column, 2D and 3D single-molecule techniques were utilized. We uncover the differences in protein motion in mobile phases, depending on salt concentration, and correlated the results to an ensemble chromatogram. We also demonstrate the importance of the combined influence of surface properties on adsorption-desorption kinetics of proteins to the stationary phase. Overall, we have shown that single-molecule methods can uncover the details of protein dynamics and transport at the nanoscale and relate them to ensemble chromatography and apply them to protein purification at-scale.